{"id":540,"date":"2023-01-17T15:20:11","date_gmt":"2023-01-17T20:20:11","guid":{"rendered":"https:\/\/rybelz.com\/ovcharenko\/?p=540"},"modified":"2023-01-19T15:18:37","modified_gmt":"2023-01-19T20:18:37","slug":"2020-katya-murkes-summer-internship-project","status":"publish","type":"post","link":"https:\/\/rybelz.com\/new-ovcharenko\/2020-katya-murkes-summer-internship-project\/","title":{"rendered":"2020 Katya Murkes Summer Internship Project"},"content":{"rendered":"

Katya, you signed up for a summer internship project that will take a lot of your time and effort. This will not be easy. Please set your expectations that there will be a lot of work to do.<\/p>\n

Please use this page as your science notebook for your summer internship \u2013 all your answers, observations, questions, and results \u2013 all should be added here on this page (you can add text and post pictures here; using this page will also teach you how to use the WordPress platform).<\/p>\n

Please start with internet research regarding the instrument you received by answering the following questions (copy\/paste will not work, you will need to perform the internet research using google.com and then write your own answers to the following questions):<\/p>\n

Model and name of the instrument:\u00a0Student school biological microscope. RM-1B Radical Instruments<\/strong><\/p>\n

What is it used for?\u00a0Microscopes are used for viewing very small objects, like insects or cells, up close.<\/strong><\/p>\n

Who needs it?\u00a0Microscopes can be used by many different people, but this one is made specifically for students.<\/strong><\/p>\n

What parts is it made of?\u00a0The microscope has an eyepiece, a body tube, and arm, a stage, 3 objective lenses, and a revolving nose piece to switch the objective lens. Also, it has stage clips to clip the viewing slides to the stage,<\/strong>\u00a0some focus adjustment knobs, a base, and a mirror<\/strong>\u00a0for illumination.<\/strong><\/p>\n

How does it work? Describe in great detail.\u00a0You look through the eyepiece and you can see the slide through<\/strong>\u00a0one of the objective lenses<\/strong>.\u00a0The mirror or light under the stage illuminates the slide. You can focus the image by turning the focus adjustment knobs on the sides of the microscope.<\/strong><\/p>\n

Were you able to find an instrument manual online?\u00a0Yes<\/strong><\/p>\n

Read the manual. Describe what was clear in the manual and what was confusing.\u00a0Everything was clear<\/strong><\/p>\n

Read the details on what is Histology, how H&E works, how to identify different type of cells and tissue (how different disease cells\/tissues look?):<\/strong><\/p>\n

Histology<\/a><\/p>\n

A Beginner\u2019s Guide to Haematoxylin and Eosin Staining<\/a><\/p>\n

Histology Guide<\/a><\/p>\n

https:\/\/histology.leeds.ac.uk\/what-is-histology\/H_and_E.php<\/a><\/p>\n

After you have completed internet research, try to turn on the instrument and see what happens. Make a picture and post it here. You may be missing power cord and\/or adapter cables (find out what cords\/cables\/adapters are missing, find missing part on Amazon or Ebay, and post Amazon\/Ebay link here, so I can get you the missing part). Some instruments been in storage and are very dusty \u2013 clean them with Windex spray and paper towels (your parents got a cleaning supplies cabinet somewhere in your house, find it).<\/p>\n

Try to operate the instrument using the manual \u2026 is it working? Do you need certain supplies to make it work? Find the catalog numbers (from the manual) of the required supplies, where from they are available, and post here links of what we need to order. Post here results of your testing, and how do you plan to move forward.<\/p>\n

This is your notebook now \u2026 write and post answers and pictures below:<\/strong><\/p>\n

Katya:<\/strong><\/p>\n

This is the microscope. It is working. There is nothing missing.<\/p>\n

\"Picture<\/p>\n

June 29, 2020<\/em><\/p>\n

HISTOLOGY: Histology, which can also be known as microanatomy, is the microscopic study of cells and tissues. Histopathology is a branch of histology that studies diseased tissues in medicine.<\/p>\n

H&E: Tissues and cells are transparent of dull grey in nature, which makes them really hard to view under a microscope. For this reason, the cells and tissues are stained with different colors. The most common staining system is H&E, which consists of the dyes Hematoxylin and Eosin.<\/p>\n

Eosin is an acidic dye which is negatively charged. Because acidic dyes bind to basic cell structures, Eosin dyes cytoplasm, which is basic, a red or pink color.<\/p>\n

Hematoxylin is a basic dye, so it stains acidic structures purple or blue. Hematoxylin is actually a dye called hematein. Hematein alone cannot stain tissues. It needs to be used together with a mordant, which is a compound. When hematein stains something, the mordant first binds to the tissue, then the hematein binds to the mordant.<\/p>\n

June 30, 2020<\/em><\/p>\n

IDENTIFYING DISEASED TISSUE: Histology can be helpful when a doctor is trying to diagnose a certain disease. Some diseases have clear visual signs of their presence, while others require non-histological forms of testing.<\/p>\n

Even though we usually can\u2019t see the virus or bacteria itself under a light microscope, we can still see the signs that the virus is present. The tissue around the area will be damaged, and certain spots could be colored differently under H&E staining. That is a spot impacted by a virus or bacteria.<\/p>\n

\"Image
A colony of bacteria on muscle tissue<\/figcaption><\/figure>\n

This is not my photo, see Introduction to histopathology<\/a>.<\/p>\n

July 1, 2020<\/em><\/p>\n

Dima, you told me to look at Maria\u2019s work on a similar project to help me figure out what to do next. I will continue to do the following:<\/p>\n

    \n
  1. Research morphology of cells (how to identify different kinds of cells)<\/li>\n
  2. Research architecture of tissues (how cells are structured in tissues)<\/li>\n
  3. learn how the structure of cells and tissues affects their function<\/li>\n
  4. Learn how cells and tissues appear normally<\/li>\n
  5. learn how cells and tissues change during disease<\/li>\n
  6. identify which slides have diseased cells and which have healthy cells<\/li>\n<\/ol>\n

    MORPHOLOGY<\/strong>:\u00a0the branch of biology that deals with the form of living organisms, and with relationships between their structures.<\/em>\u00a0(definition from Google)<\/p>\n

    July 2, 2020<\/em><\/p>\n

    \"Microscopic<\/p>\n

     <\/p>\n

     <\/p>\n

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    This is a picture I took. These are skeletal muscle cells. The little purple dots are nuclei.<\/p>\n

    July 3rd and 4th, 2020<\/em><\/p>\n

    \"Microscopic
    I think this might be cardiac muscle, but on that slide there was also some other tissue that looked different, and I\u2019m not sure what it could be.<\/figcaption><\/figure>\n

     <\/p>\n

    I think these maybe brain cells.<\/p>\n

     <\/p>\n

    \"\"<\/p>\n

    July 5, 2020<\/em><\/p>\n

    Dima, I read your instructions and will now be posting examples of each type of cell here, before I try to find them on my slides.<\/p>\n

    BRAIN CELL EXAMPLES<\/strong><\/p>\n